![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4790084:Design
Designed by: Hongyan Jing Group: iGEM23_NNU-CHINA (2023-10-11)
ASR4(node 109)/pET-22b(+)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 450
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 948
Illegal PstI site found at 450 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 450
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 450
Illegal NgoMIV site found at 143 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
The original sequence of pET-22b was downloaded from NCBI. Node 109 was constructed through Ancestral Sequence Reconstruction (ASR).